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EC Number
Purification (Commentary)
2.3.1.247
native enzyme 143fold by anion exchange chromatography
2.3.1.247
native soluble enzyme 78fold by ammonium sulfate fractionation and anion exchange chromatography, followed by ultrafiltration and adsorption chromatography
2.3.1.247
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, desalting gel filtration, anion exchange chromatography, and gel filtration
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