EC Number   |
|---|
   2.3.1.183 | - |
| 2.3.1.183 | for the purification of PAT from Pseudomonas fluorescens ammonium sulfate precipitation, a Source 15 Phenyl XK hydrophobic interaction column, and a Superdex 75 XK gel filtration column are used |
| 2.3.1.183 | recombinant enzyme from Escherichia coli by ammonium sulfate fractionation, anion exchange, hydrophobic interaction, and hydroxylapatite chromatography, followed by gel filtration, to about 90% purity |
| 2.3.1.183 | recombinant enzyme from Pseudomonas fluorescens to homogeneity by ammonium sulfate fractionation, hydrophobic interaction chromatography, and gel filtration |
| 2.3.1.183 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant enzyme from transgenic maize and rape plants by immunoaffinity chromatography, method development, overview |
| 2.3.1.183 | simple, time-saving, inexpensive and reproducible purification method employs a single chromatography step using a reactive dye resin, Reactive brown 10-agarose. Reactive brown 10 preferentially binds the phosphinothricin acetyltransferase protein, which can then be specifically released by acetyl-CoA. Phosphinothricin acetyltransferase protein can be purified to homogeneity from cottonseed with high recovery efficiency and enzymatically active |
| 2.3.1.183 | the recombinant protein is purified by Ni2+ affinity chromatography and used for the production of polyclonal antibodies, for Western blot analysis the protein is extracted from peppers |
