EC Number   |
|---|
   2.3.1.18 | affinity chromatography |
| 2.3.1.18 | K12 strain H30000 |
| 2.3.1.18 | recombinant His-tagged enzyme from Escherichia coli strain BL21-CodonPlus(DE3)RIPL by nickel affinity chromatography, tag cleavage with TEV protease, another step of nickel affinity chromatography, and gel filtration |
| 2.3.1.18 | recombinant His6-tagged wild-type and mutant enzymes by nickel affinity chromatography, ion exchange chromatography, and gel filtration |
| 2.3.1.18 | strain ML308 |
| 2.3.1.18 | using a HiTrap metal-chelating affinity chromatography column, a HiTrap desalting, and a Superdex 200 column |
| 2.3.1.18 | wild-type and mutant enzyme |
