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EC Number
Purification (Commentary)
2.2.1.10
recombinant enzyme from Escherichia coli by heat treatment and anion exchange chromatography
2.2.1.10
recombinant His-tagged ADHS from Escherichia coli strain B834-(DE3) by nickel affinity chromatography, cleavage of the tag by thrombin, and further by strepavidin affinity chromatography to eliminate thrombin, followed by gel filtration/ultrafiltration
2.2.1.10
soluble fraction of recombinant His6-tagged MJ0400 from Escherichia coli strain Rosetta(DE3) by nickel affinity chromatography to over 97% purity
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