EC Number |
---|
2.1.1.274 | by DE53 DEAE anion exchange, HAP and Mono-Q anion exchange chromatography |
2.1.1.274 | by nickel-based affinity chromatography |
2.1.1.274 | N-terminal polyhistidine-tagged SAMT protein purified by Ni2+ affinity chromatography and gel filtration chromatography using a Superdex-75 column |
2.1.1.274 | recombinant enzyme CsSAMT from Escherichia coli and from Saccharomyces cerevisiae |
2.1.1.274 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Codon Plus by metal affinity chromatography |
2.1.1.274 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
2.1.1.274 | to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography |