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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.273by nickel-based affinity chromatography 721415
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.273enzyme purified from upper and lower petal lobes of 5- to 10-day-old snapdragon flowers using DE53 anion exchange chromatography, hydrophobic interaction chromatography using Phenyl-Sepharose 6FF, and Mono-Q chromatography 724107
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.273from 5- to 10-day-old upper and lower petal lobes by DE53 anion exchange, phenyl–Sepharose 6FF and MonoQ chromatography 726156
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.273recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) Codon Plus by metal affinity chromatography 736842
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.273recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 757984
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.273to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography 723416
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