EC Number |
---|
2.1.1.160 | native enzyme 24fold from young leaves by 3 steps of ion exchange chromatography, and gel filtration |
2.1.1.160 | native enzyme 523fold from young, developing leaves by ammonium sulfate fractionation, hydroxylapatite, anion exchange, and adenosine affinity chromatography, and gel filtration to homogeneity |
2.1.1.160 | recombinant His-tagged DXMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration |
2.1.1.160 | recombinant protein |
2.1.1.160 | using the His-tagged affinity chromatography |