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Search Purification (Commentary)

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Don't show organism specific information (fast!)
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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.8.99.B1- 661641, 679756, 724941, 724983, 725700, 726026
Display the word mapDisplay the reaction diagram Show all sequences 1.8.99.B1recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21-Codon Plus (DE3)RIL by ultracentrifugation, heat treatment at 70°C for 20 min, metal chelating affinity chromatography, dialysis, anion exchange chromatography, and again dialysis 742465
Display the word mapDisplay the reaction diagram Show all sequences 1.8.99.B1wild-type enzyme and recombinant mutant enzymes C34S, C148S and C34S/C148S 725698
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