EC Number   |
|---|
   1.8.4.2 | - |
| 1.8.4.2 | anion-exchange chromatography (DEAE) and gel filtration (Superdex 75) |
| 1.8.4.2 | Ni2+-NTA affinity chromatography |
| 1.8.4.2 | nickel-nitrilotriacetic acid column chromatography |
| 1.8.4.2 | partially |
| 1.8.4.2 | recombiant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography and gel filtration |
| 1.8.4.2 | recombiant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration |
| 1.8.4.2 | recombinant enzyme |
| 1.8.4.2 | recombinant enzyme from periplasm |
| 1.8.4.2 | recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain XL-1 Blue by nickel affinity chromatography |
