EC Number   |
Reference |
|---|
   1.8.1.B1 | - |
659902, 687495, 687730, 689706, 742460, 763800, 763856, 763906, 764019, 764219 |
| 1.8.1.B1 | 2',5'-ADP Sepharose column chromatography and Sephadex G-25 gel filtration |
724324 |
| 1.8.1.B1 | ADP-agarose column chromatography |
726293 |
| 1.8.1.B1 | ammonium sulfate precipitation, Ni-NTA column chromatography, GSH-Sepharose column chromatography, and 2',5'-ADP-Sepharose column chromatography |
724926 |
| 1.8.1.B1 | DEAE-Sephacel gel filtration, hydroxyapatite chromatography column chromatography, and Cibacron blue chromatography column chromatography |
725773 |
| 1.8.1.B1 | Ni-NTA column chromatography |
725454 |
| 1.8.1.B1 | nickel affinity column chromatography |
726093 |
| 1.8.1.B1 | recombinant enzyme |
688886 |
| 1.8.1.B1 | recombinantly produced human enzyme (hTGR), the Sec->Cys mutant (hTGRU642C) of the full-length protein, the isolated TrxR domain (hTGR151-643) and the Grx domain containing a monothiol active site (hTGR1-150) are produced |
764653 |
| 1.8.1.B1 | small amounts of the soluble enzyme are obtained by purifying it from a large quantity of cells, in which protein synthesis is induced slowly for a period of 24 h at a low temperature |
685060 |
