EC Number |
---|
1.7.2.3 | - |
1.7.2.3 | HiTrap Chelating HP column chromatography |
1.7.2.3 | HiTrap Q FF column chromatography |
1.7.2.3 | Ni-NTA column chromatography and Superdex 200 gel filtration |
1.7.2.3 | of 2 enzyme forms, using ammonium sulfate treatment, ion exchange buffer treatment, column chromatography on DEAE-Sepharose CL6B and hydroxyapatite and gel filtration on Sephacryl S-300 |
1.7.2.3 | recombinant His-tagged enzyme by nickel affinity and anion exchange chromatography |
1.7.2.3 | recombinant His-tagged enzyme from strain BL21(DE3) to near homogeneity |
1.7.2.3 | recombinant His-tagged monomeric, dimeric, and trimeric forms from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, and gel filtration for the the monomeric form |
1.7.2.3 | using ammonium sulfate treatment and chromatography on Sephacryl S-200 column and Mono-Q column |
1.7.2.3 | using heat treatment, ammonium sulfate precipitation and chromatography on Bio-Gel A-1.5m, DEAE-cellulose and reactive blue-agarose |