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Search Purification (Commentary)

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Results 1 - 8 of 8
EC Number Purification (Commentary) Reference
Display the reaction diagram Show all sequences 1.7.1.17- 742823, 744571, 765245
Display the reaction diagram Show all sequences 1.7.1.17native enzyme 4500fold by anion exchange chromatography, Blue Sepharose affinity chromtography, gel filtration, hydroxyapatite chromatography, again anion exchange chromatography and hydrophobic interaction chromatography. Recombinant enzyme AcpD from Escherichia coli, strain JM109 by anion exchange chromatography, Blue Sepharose affinity chromtography, gel filtration, and chromatography on GIGAPITE resin, the purified gene product exhibits activity corresponding to that of the native azoreductase 742823
Display the reaction diagram Show all sequences 1.7.1.17Ni-NTA column chromatography 764598
Display the reaction diagram Show all sequences 1.7.1.17Ni-Sepharose column chromatography 763801
Display the reaction diagram Show all sequences 1.7.1.17recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 743106
Display the reaction diagram Show all sequences 1.7.1.17recombinant His-tagged enzyme from Escherichia coli strain XL1-Blue by nickel affinity chromatography 743185
Display the reaction diagram Show all sequences 1.7.1.17recombinant His-tagged wild-type and mutant AzoR enzymes from Escherichia coli BL21(DE3) by nickel affinity chromatography 742834
Display the reaction diagram Show all sequences 1.7.1.17recombinant His-tagged wild-type and mutant enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography, dialysis, and ultrafiltration 763965
Results 1 - 8 of 8