EC Number   |
|---|
    1.3.98.1 | - |
| 1.3.98.1 | acid treatment, DE-52-, Matrex Orange A-, Matrex Blue A column chromatography |
| 1.3.98.1 | ammonium sulfate precipitation and Q-Sepharose column chromatography |
| 1.3.98.1 | by ion-exchange chromatography |
| 1.3.98.1 | centrifugation after cell disruption, separation from the NADP-linked dihydroorotic dehydrogenase |
| 1.3.98.1 | ion-exchange, gel filtration, ammonium sulfate precipitation |
| 1.3.98.1 | native enzyme from the cytosolic fraction by gel filtration and immunoaffinity chromatography, recombinant His-tagged enzyme by nickel affinity chromatography to homogeneity and subsequent clevage of the tag by thrombin |
| 1.3.98.1 | Ni2+-NTA agarose chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration |
| 1.3.98.1 | Orange-A column, reverse-affinity step, Mono Q column |
| 1.3.98.1 | partial |
