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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.3.7.15- 725369
Display the word mapDisplay the reaction diagram Show all sequences 1.3.7.15copurification of recombinant GST-tagged BchX and BchY from Escherichia coli by glutathione affinity chromatography 762912
Display the word mapDisplay the reaction diagram Show all sequences 1.3.7.15Ni2+-loaded chelating Sepharose column chromatography 738644
Display the word mapDisplay the reaction diagram Show all sequences 1.3.7.15recombinant N-terminally His6-tagged BchY and untagged BchZ as a subcomplex, and N-terminally thioredoxin/His/S-tagged BchX2, from Escherichia coli strain BL21(DE3) by nickel affinity chromatography under strict anaerobic conditions (i.e. in an anaerobic chamber), which is essential for the preservation of enzymatic activities. Method evaluation, overview 763435
Display the word mapDisplay the reaction diagram Show all sequences 1.3.7.15Strep-Tactin, column chromatography 737615
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