EC Number |
Reference |
---|
1.3.1.98 | - |
286059, 286060, 286063, 286064, 286065, 286066, 286068, 286070 |
1.3.1.98 | ammonium sulfate precipitation, Q-Sepharose column chromatography, and Superdex 200 gel filtration |
722831 |
1.3.1.98 | Ni-NTA agarose column chromatography |
722410 |
1.3.1.98 | recombinant enzyme from strain BL21(DE3) by ammonium sulfate fractionation, hydrophobic interaction and ion exchange chromatography to homogeneity |
669810 |
1.3.1.98 | recombinant His-tagged enzyme from Escherichia coli K12S by nickel affinity chromatography |
286071 |
1.3.1.98 | recombinant MurB from Escherichia coli strain BL21(DE3) |
689925 |
1.3.1.98 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, followed by a second step of affinity chromatography to remove uncleaved protein and the His6-tagged TEV protease, further purification by preparation scale gel filtration, and ultrafiltration |
746242 |
1.3.1.98 | recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) to homogeneity, native wild-type enzyme from Staphylococcus aureus 10fold by ammonium sulfate precipitation and two steps of anion exchange chromatography |
669466 |