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Search Purification (Commentary)

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Results 1 - 8 of 8
EC Number Purification (Commentary) Reference
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98- 286059, 286060, 286063, 286064, 286065, 286066, 286068, 286070
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98ammonium sulfate precipitation, Q-Sepharose column chromatography, and Superdex 200 gel filtration 722831
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98Ni-NTA agarose column chromatography 722410
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98recombinant enzyme from strain BL21(DE3) by ammonium sulfate fractionation, hydrophobic interaction and ion exchange chromatography to homogeneity 669810
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98recombinant His-tagged enzyme from Escherichia coli K12S by nickel affinity chromatography 286071
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98recombinant MurB from Escherichia coli strain BL21(DE3) 689925
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage by TEV protease, followed by a second step of affinity chromatography to remove uncleaved protein and the His6-tagged TEV protease, further purification by preparation scale gel filtration, and ultrafiltration 746242
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) to homogeneity, native wild-type enzyme from Staphylococcus aureus 10fold by ammonium sulfate precipitation and two steps of anion exchange chromatography 669466
Results 1 - 8 of 8