EC Number |
---|
1.3.1.34 | dye-ligand chromatography, general ligand affinity-chromatography |
1.3.1.34 | gel filtration |
1.3.1.34 | ion-exchange chromatography |
1.3.1.34 | ion-exchange chromatography or dye-ligand chromatography, affinity chromatography |
1.3.1.34 | Ni-NTA chromatography |
1.3.1.34 | NTA-Ni2+ agarose column chromatography |
1.3.1.34 | partial, solubilization of enzyme using Triton X-100 from heart mitochondria |
1.3.1.34 | recombinant enzyme from strain BL21(DE3) via DEAE ion exchange and affinity chromatography |
1.3.1.34 | recombinant His-tagged truncated enzyme from Escherichia coli strain BL21(DE3) by one-step nickel affinity chromatography, to over 95% purity |