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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Show all pathways known for 1.2.1.85Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.85ammonium sulfate fractionation, TSKgel DEAE-5PW column chromatography, Affigel-Blue affinity chromatography, and TSKgel Phenyl-5PW column chromatography 390225
Show all pathways known for 1.2.1.85Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.85recombinant soluble N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, tag cleavage by TEV protease, and another nickel affinity chromatography step, followed by gel filtration and ultrafiltration 741776
Show all pathways known for 1.2.1.85Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.85streptomycin sulfate precipitation, ammonium sulfate precipitation, DE52 cellulose column chromatography, DEAE-cellulofine column chromatography, and DEAE-Toyopearl column chromatography 672682
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