EC Number   |
|---|
    1.2.1.8 | - |
| 1.2.1.8 | 2 enzyme forms: BADH I and BADH II |
| 1.2.1.8 | aldehyde dehydrogenase E3 isozyme is a betaine aldehyde dehydrogenase |
| 1.2.1.8 | by a two step procedure |
| 1.2.1.8 | For purification of the recombinant protein, cells of 500 ml culture are harvested. Pellet is suspended in lysis buffer and suspension was incubated. Following sonication, suspension is centrifuged. Clear supernatant is collected and loaded onto a Glutathione-Agarose column equilibrated with buffer (20 mM Tris-HCl (pH 8.0), 5.0 mM EDTA). The recombinant protein is eluted with elution buffer (20 mM Tris-HCl (pH 8.0), 5.0 mM EDTA, and 1.0 mM reduced glutathione). Eluted protein is collected and the purity is analyzed by 12% SDS-PAGE. To remove the GST moiety, the recombinant proteins are digested with PreScission protease. |
| 1.2.1.8 | His-Tag affinity chromatography |
| 1.2.1.8 | Ni Sepharose affinity column chromatography, and gel filtration |
| 1.2.1.8 | Ni-NTA column chromatography |
| 1.2.1.8 | Ni-NTA column chromatography and Superdex 200 gel filtration |
| 1.2.1.8 | on GSTrap FF columns |
