EC Number   |
|---|
    1.2.1.27 | - |
| 1.2.1.27 | MSDH purified by ammonium sulfate fractionation, 40-80%, and gel filtration on a ACA 34 resin |
| 1.2.1.27 | partial |
| 1.2.1.27 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration to over 95% purity |
| 1.2.1.27 | recombinant MSDH |
| 1.2.1.27 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3). Among the twelve molecules in the asymmetric unit, six subunits complexed with NAD+ |
| 1.2.1.27 | to apparent homogeneity using heat precipitation at 80°C for 20 min, ion exchange chromatography and gel filtration |
