EC Number |
---|
1.2.1.13 | - |
1.2.1.13 | 2 isoenzymes: 1 and 2 |
1.2.1.13 | 3 enzyme forms: GAPDH I, GAPDH II, GAPDH III |
1.2.1.13 | by Ni2+ chelate chromatography and pH-shift under denaturating conditions |
1.2.1.13 | cell-free preparations for measuring the enzyme activities are obtained |
1.2.1.13 | DEAE Trisacryl column chromatography |
1.2.1.13 | gel filtration |
1.2.1.13 | heat treatment at 80°C is an effective first step in the purification of these recombinant enzymes from extracts of the Escherichia coli host |
1.2.1.13 | HisTrap column chromatography and Superdex 200 gel filtration |
1.2.1.13 | native and recombinant enzyme |