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EC Number
Purification (Commentary)
1.16.1.8
after expression by baculovirus-infected insect cells. the flavin mononuleotide cofactor dissociates readily from enzyme upon dilution, hence it is important to keep the concentration high during purification
1.16.1.8
recombinant enzyme
1.16.1.8
recombinant GST-tagged full-length enzyme and flavin-binding domain by glutathione affinity and anion exchange chromatography, followed by ultrafiltration
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