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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.16.1.8after expression by baculovirus-infected insect cells. the flavin mononuleotide cofactor dissociates readily from enzyme upon dilution, hence it is important to keep the concentration high during purification
Display the word mapDisplay the reaction diagram Show all sequences 1.16.1.8recombinant enzyme
Display the word mapDisplay the reaction diagram Show all sequences 1.16.1.8recombinant GST-tagged full-length enzyme and flavin-binding domain by glutathione affinity and anion exchange chromatography, followed by ultrafiltration
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