EC Number   |
|---|
   1.14.99.66 | LSD1 in the histone demethylase complex also containing CoREST, HDAC1, and HDAC2 |
| 1.14.99.66 | recombinant |
| 1.14.99.66 | recombinant FLAG-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by M2 affinity chromatography, recombinant FLAG-tagged wild-type enzyme from insect Sf21 cells by M2 affinity chromatography |
| 1.14.99.66 | recombinant Flag-tagged wild-type KDM1B and mutant KDM1B from HEK 293T cells to near homogeneity |
| 1.14.99.66 | recombinant full-length His6-tagged LSD1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis |
| 1.14.99.66 | recombinant His-tagged LSD1 from Escherichia coli by nickel and heparin affinity chromatography |
| 1.14.99.66 | recombinant His6-tagged wild-type LSD2 and truncated mutants from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography, followed by ultra- and gel filtration |
| 1.14.99.66 | recombinant N-terminally His-tagged enzyme by nickel affinity chromatography from Escherichia coli BL21(DE3), or by ammonium sulfate fractionation and anion exchange chromatography, recombinant N-terminally truncated GST-tagged LSD1 by glutathione affinity chromatography |
| 1.14.99.66 | two LSD1 truncated forms, lacking the first 157 and 184 amino acids, respectively |
