Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search Purification (Commentary)

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 5 of 5
EC Number Purification (Commentary)
Show all pathways known for 1.14.99.39Display the word mapDisplay the reaction diagram Show all sequences 1.14.99.39-
Show all pathways known for 1.14.99.39Display the word mapDisplay the reaction diagram Show all sequences 1.14.99.39native enzyme 9.7fold by by anion-exchange chromatography and gel filtration as membrane-bound monomer
Show all pathways known for 1.14.99.39Display the word mapDisplay the reaction diagram Show all sequences 1.14.99.39native enzyme by anion exchange chromatography and gel filtration
Show all pathways known for 1.14.99.39Display the word mapDisplay the reaction diagram Show all sequences 1.14.99.39recombinant C-terminally Strep-tagged protein, comprising amino acids 31-186, from Escherichia coli strain Rosetta-2 (DE3) by affinity chromatography, ultrafiltration, and gel filtration
Show all pathways known for 1.14.99.39Display the word mapDisplay the reaction diagram Show all sequences 1.14.99.39the free ethynyl group of the inactive enzyme-inactivator adduct is conjugated with either a visualization tag (e.g., Alexa Fluor 647 azide) or an affinity purification tag (e.g., biotin-azide) using a copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. The resulting enzyme-probe-tag conjugant can then either be (i) visualized using IR fluorescence in SDS-PAGE or (ii) enriched by affinity chromatography, tryptically digested, and identified by LC-MS/MS
Results 1 - 5 of 5