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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the reaction diagram Show all sequences 1.14.19.8Ni2+-NTA resin column chromatography 719654
Display the reaction diagram Show all sequences 1.14.19.8recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 by nickel affinity chromatography, tag cleavage by thrombin, another step of nickel affinity chromatography, and gel filtration of the eluate, followed by ultrafiltration 745166
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