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EC Number
Purification (Commentary)
1.14.15.16
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1.14.15.16
recombinant active His-tagged enzyme from Escherichia coli membranes by nickel affinity and hydrophobic interaction chromatography and dialysis, adrenodoxin-affinity chromatography is not successful
1.14.15.16
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain DH5alpha F'IQ by nickel affinity chromatography and gel filtration, copurification with added recombinant bovine adrenodoxin for enzyme-cofctor complex preparation
1.14.15.16
recombinant N-terminally maltose-binding-protein fused enzyme from Escherichia coli by affinity chromatography to homogeneity
1.14.15.16
Talon metal affinity column chromatography
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