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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.14.14.83native cholate-solubilized G10H about 8.3fold from suspension cell membranes by anion exchange and hydroxyapatite chromatography, and 2-aminooctyl agarose and a second step of hydrophobic interaction chromatography to homogeneity. Separation from the NADPH-cytochrome P-450 reductase, EC 1.6.2.4
Display the word mapDisplay the reaction diagram Show all sequences 1.14.14.83native enzyme about 12fold from membranes by anion exchange and omega-amino octyl agarose chromatography, followed by hydophobic interaction chromatography to homogeneity
Display the word mapDisplay the reaction diagram Show all sequences 1.14.14.83solubilization by treatment with sodium cholate, Renex-30, or Lubrol-WX
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