1.14.13.69 | recombinant StrepII-tagged oxygenase component IsoABE from Rhodococcus sp. strain AD45 and recombinant N-terminally His6-tagged IsoC from Escherichia coli both by affinity chromatography and gel filtration, the latter to over 90% purity. Recombinant StrepII-tagged IsoD from Escherichia coli strain Rosetta2 by affinity, anion exchange, and hydrophobic interaction chromatography, and gel filtration, the N-terminal methionine of IsoD has been removed |