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Search Purification (Commentary)

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Results 1 - 8 of 8
EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.9-
Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.9recombinant enzyme
Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.9recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.9recombinant MBP-tagged enzyme from Escherichia coli BL21(DE3)pLysS by amylose affinity chromatography
Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.9Sephadex G25 column gel filtration
Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.9shock frozen fruits are ground in a mill, leaves are ground in liquid nitrogen in a mortar, protocol 1: plant material is homogenized in a mortar with quartz sand and Polyclar AT with extraction buffer (0.1 M Tris-HCl, pH 7.5, containing 0.4% sodium ascorbate) and centrifuged, or protocol 2 (optimized for polyphenol-rich tissues): plant material is homogenized with Polyclar AT in a mortar, transferred to a falcon tube containing Dowex in buffer (0.7 M KH2PO4/K2HPO4, pH 8.0, containing 0.4 M sucrose, 0.4 M sodium ascorbate, 1 mM CaCl2, 30 mM EDTA, 50 mM cysteine, 50 mM DIECA, 1.5% PEG 20000, and 0.1% BSA, kept under nitrogen atmosphere after removing oxygen by boiling), homogenate is filtered (glass wool) and centrifuged, supernatants obtained with both protocols are cleared of low molecular compounds by a Sephadex G25 gel chromatography column
Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.9shock frozen fruits are ground in a mill, leaves are ground in liquid nitrogen in a mortar, protocol 1: plant material is homogenized in a mortar with quartz sand and Polyclar AT with extraction buffer (0.1 M Tris-HCl, pH 7.5, containing 0.4% sodium ascorbate) and centrifuged, or protocol 2 (optimized for polyphenol-rich tissues): plant material is homogenized with Polyclar AT in a mortar, transferred to a falcon tube containing Dowex in buffer (0.7 M KH2PO4/K2HPO4, pH 8.0, containing 0.4 M sucrose, 0.4 M sodium ascorbate, 1 mM CaCl2, 30mM EDTA, 50 mM cysteine, 50 mM DIECA, 1.5% PEG 20000, and 0.1% BSA, kept under nitrogen atmosphere after removing oxygen by boiling), homogenate is filtered (glass wool) and centrifuged, supernatants obtained with both protocols are cleared of low molecular compounds by a Sephadex G25 gel chromatography column
Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.9wild-type and mutant enzymes
Results 1 - 8 of 8