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Search Purification (Commentary)

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Results 1 - 4 of 4
EC Number Purification (Commentary) Reference
Show all pathways known for 1.14.11.8Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.8- 652164, 764104
Show all pathways known for 1.14.11.8Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.8partial 7020
Show all pathways known for 1.14.11.8Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.8recombinant active and stable His- and MBP-tagged TMLH from Escherichia coli strain BL21-AI by nickel or amylose affinity chromatography. It is possible that the His6-tag at the N-terminus of the fusion protein is masked and cannot be accessed by the Ni-beads. Thus, MBP affinity purification is selected as a first purification step, followed by gel filtration, His6-MBP-tag cleavage by recombinant TEV protease 746366
Show all pathways known for 1.14.11.8Display the word mapDisplay the reaction diagram Show all sequences 1.14.11.8recombinant MBP-taged enzyme by affinity chromatography, tag cleavage by rTEV protease, and gel filtration 744681
Results 1 - 4 of 4