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Search Purification (Commentary)

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EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.66- 718555, 718831
Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.66ligandation with 4-hydroxybenzoate prevents the enzyme from irreversible inactivation 719714
Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.66recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 745331
Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.66recombinant N-terminally His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, tag removal by rTEV protease cleavage, over 95% purity. No enzyme activity can be detected with purified YaiA, even when it is purified in the presence of 4-hydrobenzoate 745802
Display the word mapDisplay the reaction diagram Show all sequences 1.13.11.66using Ni-NTA chromatography 719179
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