EC Number   |
|---|
    1.13.11.55 | - |
| 1.13.11.55 | heat treatment, DEAE-52 anion exchange and Sephadex G-200 chromatography |
| 1.13.11.55 | one-step procedure over Strep-Tactin columns |
| 1.13.11.55 | purified by gel filtration |
| 1.13.11.55 | recombinant protein, by heat treatment and chromatography steps with four different columns |
| 1.13.11.55 | recombinant wild-type and mutant enzymes from Escherichia coli |
| 1.13.11.55 | single-step purification of the proteins is obtained via fused His or Strep tags |
| 1.13.11.55 | solubilized recombinant wild-type and mutant enzymes from Escherichia coli strain B21 inclusion bodies by nickel affinity chromatography, and refolded by a four-step dialysis |
| 1.13.11.55 | sucrose density gradient centrifugation and preparative, non-denaturing PAGE |
| 1.13.11.55 | Superdex 200 gel-filtration |
