EC Number |
---|
1.1.5.9 | - |
1.1.5.9 | by DEAE-5PW and Superdex-200 10/300GL column chromatography |
1.1.5.9 | DEAE-5PW column chromatography and Superdex-200 gel filtration |
1.1.5.9 | HisTrap nickel affinity chromatography |
1.1.5.9 | isopropanol precipitation and nickel affinity column chromatography |
1.1.5.9 | native extracellular enzyme 23.7fold from culture supernatant by anion exchange and hydrophobic interaction chromatography, ammonium sulfate fractionation, and ultrafiltration |
1.1.5.9 | Ni-NTA agarose column chromatography |
1.1.5.9 | recombinant enzyme from Escherichia coli strain BL21RIL |
1.1.5.9 | recombinant enzyme from Pichia pastoris strain X-33 5.1fold by hydrophobic interaction and anion exchange chromatography |
1.1.5.9 | recombinant enzyme from Pichia pastoris to homogeneity |