EC Number |
---|
1.1.3.15 | - |
1.1.3.15 | centrifugation, washing with 10 mM potassium phosphate buffer, pH 6.5, stored at -20°C, resuspended with same buffer, treated with lysozyme followed by disruption with glass beads in a bead mill, centrifugation, supernatants used for enzyme assays |
1.1.3.15 | native enzyme from leaves 207fold by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and again gel filtration |
1.1.3.15 | native enzyme from leaves to homogeneity |
1.1.3.15 | native enzyme from maize leaf bundle sheath 46.6fold by gel filtration, anion exchange chromatography, and again gel filtration. Native enzyme 10.5fold from leaf mesophyll, by ammonium sulfate fractionation, gel filtration, anion exchange chromatography, and again gel filtration |
1.1.3.15 | native enzyme, subcellular fractionation |
1.1.3.15 | near homogeneity, precipitation techniques |
1.1.3.15 | partial |
1.1.3.15 | partial, isoenzymes A and B |
1.1.3.15 | purification via dye affinity chromatography |