EC Number   |
|---|
    1.1.1.6 | - |
| 1.1.1.6 | gene gldA, recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, and dialysis |
| 1.1.1.6 | GST-tagged protein |
| 1.1.1.6 | HisTrap column chromatography and Superdex 200 gel filtration |
| 1.1.1.6 | immobilized metal ion affinity chromatography (Co2+) |
| 1.1.1.6 | immobilized metal ion affinity chromatography (Ni2+) |
| 1.1.1.6 | purified by Ni-affinity chromatography |
| 1.1.1.6 | purified with 25fold purification and 68% yield using a single chromatographic step |
| 1.1.1.6 | purity estimated from SDS-PAGE: 95% |
| 1.1.1.6 | recombinant His-tagged chimeric enzyme GDH-NOX from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
