EC Number |
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1.1.1.48 | 12% (w/v) ammonium sulfate precipitation and 14% (w/v) polyethylene glycol 2000 precipitation at pH 7.5 |
1.1.1.48 | ammonium sulfate, CM-Sephadex, DEAE-Sephadex, hydroxyapatite, disc electrophoresis |
1.1.1.48 | ammonium sulfate, DEAE-Sepharose, FPLC-Superose 12, beta-galactosidase-galactose dehydrogenase fusion enzyme |
1.1.1.48 | ammonium sulfate, heat, phosphate precipitation, DEAE-cellulose, hydroxyapatite |
1.1.1.48 | aqueous two-phase system composed of 12% (w/v) PEG-6000, 15% (w/v) K2HPO4 with pH 7.5 at 25°C |
1.1.1.48 | by affinity chromatography on chimeric mimodye-ligand gel resins, method evaluation |
1.1.1.48 | Mono Q column, partially purified enzyme |