EC Number |
---|
1.1.1.274 | - |
1.1.1.274 | ammonium sulfate, DEAE-Sepharose, Matrix Red-A, Sephacryl S-200, hypatite C |
1.1.1.274 | DEAE-cellulose, Cibacron blue F3GA agarose, gel filtration |
1.1.1.274 | DEAE-Sepharose column chromatography and Superdex 200 gel filtration |
1.1.1.274 | native enzyme from strain BL21(DE3), 730fold to homogeneity by ammonium sulfate fractionation, DEAE ion exchange and Red-A affinity chromatography, ultrafiltration, hypatite C-resin chromatography, and a second ultrafiltration step |
1.1.1.274 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography |
1.1.1.274 | recombinant isoenzyme A |
1.1.1.274 | recombinant isoenzyme A, Red A dye-affinity, anion exchange, gel filtration |
1.1.1.274 | recombinant isoenzymes A and B, affinity chitin-binding tag |