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Search Purification (Commentary)

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EC Number Purification (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.214form A and form B of the enzyme obtained separated by rupture, centrifugation and column chromatography on DEAE-cellulose and hydroxylapatite
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.214recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography, and tag cleavage by thrombin, followed by anion exchange chromatography, gel filtration, and dialysis
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.214recombinant protein
Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.214using affinity chromatography on Red-Agarose and several subsequent ion exchange steps
Results 1 - 4 of 4