EC Number   |
|---|
    1.1.1.213 | - |
| 1.1.1.213 | ammonium sulfate precipitation, Ca2(PO4)2 gel extraction, ethanol precipitation |
| 1.1.1.213 | ammonium sulfate precipitation, Q-Sepharose, Red A, HA-Ultrogel, Sephadex G-100, copurification with NAD+ dependent enzyme |
| 1.1.1.213 | anion exchange and Blue Sepharose column |
| 1.1.1.213 | CaCl2 and ammonium sulfate fractionation, DEAE-cellulose- and hydroxyapatite chromatography, chromatofocusing, Sephadex G-100 |
| 1.1.1.213 | Centriprep-10 concentration, DEAE-HPLC |
| 1.1.1.213 | N167A chromatofocusing and gel filtration introduced between the to others chromatography steps |
| 1.1.1.213 | native enzyme 73.3fold from liver by ammonium sulfate, anion-exchange chromatography, gel filtration, and affinity chromatography |
| 1.1.1.213 | purification of recombinant wild type and mutants of AKR1C9 |
| 1.1.1.213 | recombinant enzyme from Escherichia coli strain C41(DE3) in a successive chromatographic procedure, to homogeneity |
