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EC Number Purification (Commentary) Reference
Show all pathways known for 1.1.1.198Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.198ammonium sulfate precipitation, phenyl Sepharose column chromatography and Q-Sepharose column chromatography 762321
Show all pathways known for 1.1.1.198Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.198ammonium sulfate saturation, phenyl Sepharose column chromatography, and Q Sepharose column chromatography 760303
Show all pathways known for 1.1.1.198Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.198His-Trap column chromatography 760892
Show all pathways known for 1.1.1.198Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.198HisTrap column chromatography 760425
Show all pathways known for 1.1.1.198Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.198near homogeneity, chromatography techniques, affinity chromatography 286163
Show all pathways known for 1.1.1.198Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.198Ni-NTA affinity column chromatography 762115, 762171
Show all pathways known for 1.1.1.198Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.198partial 286162
Show all pathways known for 1.1.1.198Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.198partial, using Phenyl-Sepharose hydrophobic interaction, Q-Sepharose Fast Flow and Mono-Q anion-exchange columns 761009
Show all pathways known for 1.1.1.198Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.198phenyl-Sepharose column chromatography, Q-Sepharose column chromatography and MonoQ column chromatography 761009
Show all pathways known for 1.1.1.198Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.198production of enzyme in the form of inclusion body in Escherichia coli. The refolded BDH1 tends to precipitate. Insoluble recombinant BDH1 is converted into a soluble form by adding glycerol in LB medium 762321
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