EC Number   |
|---|
   1.1.1.105 | immobilized metal ion affinity chromatography |
| 1.1.1.105 | Ni2+-NTA resin column chromatography |
| 1.1.1.105 | preparation of RoDH-4 contains a minor contaminating protein band. Therefore, the exact concentration of RoDH-4 is determined based on the calibration curve constructed from several concentrations of BSA loaded on to the same SDS/PAGE gel |
| 1.1.1.105 | RDH1 activity during purification is maintained only with phosphotidylcholine |
| 1.1.1.105 | recombinant enzyme |
| 1.1.1.105 | Sephacryl S300 gel filtration |
| 1.1.1.105 | solubilized enzyme is extremely unstable and loses almost 80% of the initial activity even at partially purified stage, purification uses resistance of enzyme to proteolysis at 4°C in the presence of 1% Triton |
