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Search Purification (Commentary)

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EC Number
Commentary
Reference
a complex with 4 activities predicted to catalyze RNA editing: gRNA-directed endonuclease, terminal uridyl transferase, 3' U-specific endonuclease, RNA ligase
copurifies with 5'-hydroxyl polynucleotide kinase
editing complex
histidine-tagged
large-scale
mutant enzymes
mutant enzymes, expressed in bacteria
Ni-NTA agarose column chromatography, and DEAE Sephacel gel filtration
produced in Escherichia coli
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