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Purification (Commentary)
recombinant coexpression of tagged OleABCD proteins in Escherichia coli and purification over His6 and FLAG columns results in OleA separating, while OleBCD purifies together, irrespective of which of the four Ole proteins is tagged. Hydrocarbon biosynthetic activity of copurified OleBCD assemblies can be reconstituted by adding separately purified OleA
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