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Search Purification (Commentary)

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Results 1 - 9 of 9
EC Number
His-bind Ni2+ column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
mutant enzyme Y92C and Y92A
native and recombinant enzyme
Ni-NTA column chromatography, HiTrap Q column chromatography, and Superdex 26/60 gel filtration
Proteins are extracted from the inclusion bodies and purified following established protocols, each enzyme is pure as judged by SDS-gel electrophoresis
recombinant enzyme
using DE52 anion exchange resin, a methyl HIC, a Sephadex G-25, and a High Q column
wild-type and mutant enzymes
Results 1 - 9 of 9