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EC Number
Commentary
Reference
208fold, solubilized with octyl beta-glucoside
partial, 1306fold, solubilized with beta-octyl glucoside
recombinant enzyme from Escherichia coli
recombinant enzyme mutants V41Y and A134F to homogeneity
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) in an anaerobic chamber, further by metal affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration, followed by ultrafiltration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and dialysis
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by anion exchange chromatography and dialysis
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, removal of the tag by thrombin, and gel filtration
Results 1 - 10 of 11 > >>