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EC Number
Purification (Commentary)
recombinant C-terminally His6-tagged full-length enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, recombinant N-terminal domain of ScGS (residues 1-366) from Escherichia coli strain BL21(DE3)pLysS by ammonium sulfate fractionation, adsorption chromatography on methyl resin, gel filtration, and ultrafiltartion
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