expressed in delayed ripening tomato plants
native enzyme by ammonium sulfate fractionation, and anion exchange chromatography
Ni-NTA agarose column chromatography
recombinant His-tagged enzyme from Escherichia coli strain Rosetta by metal chelate affinity chromatography
recombinant His6-tagged ACCD from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Recombinant protein expressed with an N-terminal 6xHis-tag is purified under native/non-denaturing conditions using Ni-NTA Superflow resin.