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Search Purification (Commentary)

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Results 1 - 9 of 9
EC Number
Commentary
Reference
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephacryl S-300 gel filtration
cells of bacteria culture are disrupted and centrifugation and the enzyme in the soluble fraction is purified using Ni-NTA affinity chromatography
DEAE Sepharose Fast Flow column chromatography and Sephacryl S-300 gel filtration
Ni-NTA column chromatography
partial, 259fold
recombinant enzyme 2.4fold from Escherichia coli strain BL21-Gold(DE3) to near homogeneity
recombinant enzyme 3fold from Escherichia coli strain BL21-Gold(DE3) to near homogeneity; recombinant enzyme 3fold from Escherichia coli strain BL21-Gold(DE3) to near homogeneity
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Results 1 - 9 of 9