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Purification (Commentary)
ammonium sulfate precipitation
different methods, overview
glycyl endopeptidase from papaya latex is partitioned using aqueous two-phase (10% PEG 6000- 10% (NH4)2SO4) in combination with NH4(SO4)2 precipitation.The partially purified glycyl endopeptidase shows the potential in production of antioxidative gelatin hydrolysates. The enzyme fraction contain lower odorous compounds in papaya latex. The gelatin hydrolysate produced using the selected fraction have negligible odorous compounds
native enzyme from latex
native enzyme from latex by ion exchange and adsorption chromatography, and gel filtration
several ligands possible for affinity chromatography are presented, fractionation on hydrophobic and cation-exchange supports, overview
Results 1 - 7 of 7