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Search Purification (Commentary)

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EC Number
Commentary
Reference
anion exchange chromatography
by gel filtration
His-Tag affinity chromatography
native enzyme from duodenal mucosa by anion exchange and benzoamidine affinity chromatography, followed by hydrophobic interaction chromatography and gel filtration
Ni-NTA column chromatography,
Ni2+ affinity chromatography
of the recombinant fusion protein by osmotic shock technique and nickel affinity column chromatography
of the recombinant fusion protein using nickel affinity column chromatography
of the recombinant fusion protein using nickel affinity column chromatography, refolding and auto-catalytic cleavage
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