EC Number   |
|---|
   3.2.1.145 | ammonium sulfate precipitation, DEAE-Toyopearl 650-M column chromatography and Resource PHE column chromatography |
| 3.2.1.145 | DEAE-cellulose, CM-Toyopearl, hydroylapatite, electrofocusing, purified from commercial driselase |
| 3.2.1.145 | native enzyme 1.8fold from culture supernatant by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration |
| 3.2.1.145 | Ni-nitrilotriacetic acid agarose colum |
| 3.2.1.145 | Ni-NTA agarose column |
| 3.2.1.145 | recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) |
| 3.2.1.145 | recombinant enzyme 0.7fold from Pichia pastoris cell culture medium by cation exchange chromatography and gel filtration |
| 3.2.1.145 | recombinant enzyme expressed in Pichia pastoris |
| 3.2.1.145 | recombinant expression of His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration |
| 3.2.1.145 | recombinant selenmethionine-labeled Pc1,3Gal43A from Pichia pastoris by ultrafiltration, and hydrophobic interaction chromatography |
